Hamilton Neuros syringes used for intrauterine injections

into fetal brain matter to alter miRNA and lncRNA expression at embryonic day 14 in the mouse.  Fetal pups are returned to the mouse dam and then are allowed to finish developing until birth.  We will then assess the impact of altering specific miRs and lncRNAs which are known to regulate the glucocorticoid pathway.  The ncRNAs will be directly injected into the fetal brain and this must be done in a small volume so that the fetal mice will survive the procedure.  Once we have this procedure working we will train graduate students at the University of New Mexico, Neuroscience department.Learn more about Neuros syringes

Unique nutrition laboratory

The University of Minnesota features a nutrition laboratory class that is unique. Very few programs in the USA offer nutrition laboratory classes, and none are as biochemically focused as the curriculum of Professor Daniel Gallaher.  Research is being done on diet and colon cancer as well as diet and diabetes / obesity.  His Experimental Nutrition course (FScN 4613; 100 students per year) has influenced a significant number of students to change their academic direction and choose to go to graduate school for a laboratory-based research project. The food analysis course (FScN 4312) for food science majors is quite rigorous. Their advanced methods of food analysis course (FScN 4311) uses advanced instrumentation such as LC-MS and NMR. All of these are undergraduate courses, attesting to the strength of the University of Minnesota nutrition programs. The syringes from Hamilton would allow us to have sufficient high-quality syringes to dedicate the syringes to our teaching program, thereby assuring access and keeping them in good condition.

Work by a particularly bright undergraduate

student in the Behavioral Neuroscience laboratory at Boston College used a Hamilton syringe to produce neurotoxic lesions – a procedure that has long been performed by Assistant professor Michael McDannald and his laboratory. The problem with the neurotoxic lesion approach is that amygdala function is impaired for the rest of time following surgery. Thus, we cannot be certain that amygdala activity at the time of alcohol drinking is necessary. In her Honor\’s Thesis, undergraduate Elizabeth Mangone is going to use a new neural inactivation method – DREADDS – to turn \’off\’ the amygdala for only a few hours at a time. In the DREADDS method, a virus containing a designer drug activated receptor is infused specifically into the amygdala. Following recovery, the amygdala can then be turned \’off\’ by bodily injection of the designer drug. If successful, Elizabeth would be the first to show that amygdala activity is necessary for alcohol drinking at the time of drinking. In order to achieve specific virus infusions into the amygdala, an extremely accurate syringe is required. In our labs experience, only Hamilton syringes can provide this accuracy. However, in our lab each syringe is dedicated to particular neurotoxin or virus. Because DREADDs have never been employed in the lab, Elizabeth\’s project would enormously benefit from dedicated Hamilton syringes. Receipt of this grant would prove Elizabeth with a tremendous opportunity to performed cutting-edge research, make a novel contribution to the neural basis of alcohol drinking and graduate with Honors.Boston College has a very research active Behavioral Neuroscience group and nearly every lab is or is beginning to employ advanced viral techniques for manipulation of neural circuits.

Sustainable Energy and Environmental Applications

Wen Zhang, Ph.D., P.E., Nanomaterials for Sustainable Energy and Environmental Applications,  Assistant Professor Department of Civil and Environmental Engineering Director, Wen Zhang, Ph.D., P.E., The Environmental Engineering Teaching Laboratory New Jersey Institute of Technology 323 Martin Luther King Blvd. Newark, NJ 07102  Office Phone: (973) 596-5520; Fax: (973) 596-5790; Email: wzhang81@njit.edu Dr. Wen Zhang is currently the faculty member of NJIT’s Newark College of Engineering in the Department of Civil and Environmental Engineering as an assistant professor. Wen is a licensed Professional Engineer (P.E.) in environmental engineering. Since joining NJIT in fall 2012, Dr. Zhang has been actively engaged in multidisciplinary research in sustainable nanotechnology. Dr. Zhang’s research integrates nanotechnology into environmental engineering to develop innovative solutions for environmental sustainability and challenges in water-energy nexus. His PhD work has extensively focused on the environmental fate and transport of engineered nanoparticles, biological interactions, nanomaterial characterization at biointerfaces [1-7], as well as mechanisms of nanoparticle’s impacts on water disinfection [8]. Recently, Wen leads major efforts in the sustainable design of visible light-driven photocatalytic systems for harnessing solar energy, hydrogen evolution [9-13], and efficient degradation of emerging water contaminants. Moreover, he develops novel multifunctional nanomaterials for antimicrobial applications, microalgae harvesting for biofuel production [14-18].

  1. Utilization of non-hazardous materials and earth abundant elements for sustainable photocatalytic reactions to produce hydrogen and remove water contaminant.
Dr. Zhang (right) and his graduate student (liyuan Kuang; left) set up their photocatalytic reactor.
Dr. Zhang (right) and his graduate student (liyuan Kuang; left) set up their photocatalytic reactor.

“Solar-to-fuel” photocatalysis aims to transform solar energy into chemical fuels. Hydrogen (H2) is one of the target carbon-free energy fuels. Producing H/2 from an abundant, non-toxic, and clean source such as sunlight and water is an ideal sustainable solution. Thus, developing visible light-driven photocatalytic systems is increasingly important owing to the promising potential of harnessing solar energy and removing environmental pollutants concurrently. In Dr. Zhang’s research, carbon doped TiO2 was synthesized and anchored to graphene oxide (GO) to form a hybrid nanostructure. Such nanostructured photocatalysts exhibited enhanced visible light absorbance and photocatalytic H2 production rates even in the absence of noble or rare earth metals as co-catalysts. As opposed to rare earth elements, noble, or transition metals such as Pt, Pd, Ru, and Rh in the synthesis of photocatalysts, non-metal elements such as such as C, N, and S are likely the ideal options to achieve greater sustainability as they are earth abundant. For example, graphene is a unique carbonaceous nanomaterial providing a two-dimensional (2-D) sheet that was shown to greatly facilitate charge transfer and separation and thus improved photocatalytic activity. Moreover, graphene as a supporting matrix increased photocatalytic longevity and maintained good colloidal stability. Dr. Zhang’s new findings lay groundwork toward the design of sustainable and efficient hybrid photocatalytic materials for renewable energy harvesting. Novel visible light responsible materials made with earth abundant elements should also play a critical role in reaching environmental sustainability and broader applications such as antimicrobial functions, pathogen removal, and recalcitrant water contaminant removal.

  1. Implementation of green chemistry and engineering principles in biomass separation and treatment for algal biofuel production
Magnetophoretic separation of algal biomass and recovery of magnetic nanoparticles from concentrated algae.
Magnetophoretic separation of algal biomass and recovery of magnetic nanoparticles from concentrated algae.

Microalgae are not only the promising feedstock for biodiesel production, but also a water contaminant that negatively affects water quality (harmful algal bloom). Thus, efficient algal separation or removal from water is not only critical for biofuel production but also important for drinking water security. Magnetophoretic separation is a promising technology for rapid biomass separation from water. Dr. Zhang’s team performs extensive investigations of magnetic Fe3O4 nanoparticles as a core material to synthesize multiple nanocomposite materials with cationic polymers and semiconductor nanomaterials. Their research indicates magnetic nanocomposite materials could help achieve exceptional algal harvesting efficiency with competitive economic viability. More importantly, Dr. Zhang recently developed a technique based on the UV-induced surface hydrophobicity shift to recover and reuse these magnetic agents as shown in the figure above [14], which potentially reduces the algal harvesting cost. Recovery of nanoparticles from algal biomass using green chemistry methods may also find many other engineering applications that require recovery, recycle and reuse of valuable nanomaterials.

  1. The roles of Hamilton’s syringes

An integral part of Dr. Zhang’s research in the areas of photocatalysis and algal biofuel research has involved the use of gas chromatography (GC) and high performance liquid chromatography (HPLC). These techniques are to track and quantify the concentrations of vapor substances such as H2, CO2, and some volatile organic compounds (e.g., CH3CH2OH) that are used in photocatalytic reactions. The Hamilton gastight syringes (e.g., PTFE Luer Lock Syringes, Parts No. 81320, 81520, 82520) with MTB sampling valve (Parts No. 86580) have been particularly useful in the collection of air samples. The air samples can also be stored in the syringes with a Septum Adapter (Parts No. 31335) before further analysis. The Model 1725 RN syringes (Parts No.81130) show excellent features we need for air sample injection to GC. Similarly, the liquid sampling and injection into HPLC also benefit a lot from Hamilton’s syringes such as Model 1702 N syringe (Parts No. 80275) and Model 1705 N syringe (Part No. 80985) for manual injection of different liquid sample volumes. The student researchers in Dr. Zhang’s lab have benefited a lot from the Hamilton’s syringes during their experiments. In addition to research, The student researchers in Dr. Zhang’s lab have benefited a lot from the Hamilton’s syringes during their experiments. in our teaching sessions for undergraduate and high school students during the Introduction to Environmental Engineering lab sessions and summer research programs. We demonstrated the vapor and liquid sampling to the students and the simple operation of syringes has greatly facilitated the teaching and learning processes. There is no doubt that the work being carried out by Dr. Zhang and his team at the Department of Civil and Environmental Engineering fully embraces the School’s mission of broadening research and education in sustainability. We are so happy that Hamilton and its products are helping them achieve that goal. We firmly believe that the industrial engagement and support toward academic researchers will lead more beneficial and synergistic outcomes toward building a more sustainable society.   References

  1. Li, Y., Niu, J., Zhang, W., Zhang, L. and Shang, E. Influence of Aqueous Media on the ROS-Mediated Toxicity of ZnO Nanoparticles toward Green Fluorescent Protein-Expressing Escherichia coli under UV-365 Irradiation. langmuir, 2014, 30, 2852-2862.
  2. Zhang, W., Li, Y., Niu, J. and Chen, Y. Photogeneration of reactive oxygen species on uncoated silver, gold, nickel, and silicon nanoparticles and their antibacterial effects. Langmuir, 2013, 29, 4647-4651.
  3. Li, Y., Zhang, W., Niu, J. and Chen, Y. Surface coating–dependent dissolution, aggregation, and ROS generation of silver nanoparticles under different irradiation conditions. Environmental Science & Technology, 2013, 47, 10293–10301.
  4. Zhang, W., Hughes, J. and Chen, Y. Impacts of hematite nanoparticle exposure on biomechanical, adhesive, and surface electrical properties of E. coli cells Applied and environmental microbiology, 2012, 78, 3905-3915.
  5. Zhang, W., Rittmann, B. and Chen, Y. Size effects on adsorption of hematite nanoparticles on E. coli cells. Environ. Sci. Technol., 2011, 45, 2172-2178.
  6. Zhang, W., Stack, A.G. and Chen, Y. Interaction force measurement between E. coli cells and nanoparticles immobilized surfaces by using AFM. Colloids Surf., B, 2010, 82, 316-324
  7. Zhang, W., Kalive, M., Capco, D.G. and Chen, Y. Adsorption of hematite nanoparticles onto Caco-2 cells and the cellular impairments: effect of particle size. Nanotechnology, 2010, 21, 355103.
  8. Zhang, W. and Zhang, X. Adsorption of MS2 on oxide nanoparticles affects chlorine disinfection and solar inactivation. Water Research, 2015, 69, 59-67.
  9. Zhang, G., Zhang, W., Minakata, D., Wang, P., Chen, Y. and Crittenden, J. Efficient photocatalytic H2 production using visible-light irradiation and (CuAg)xIn2xZn2(1 − 2x)S2 photocatalysts with tunable band gaps. International Journal of Energy Research, 2014, 38, 1513-1521.
  10. Zhang, G., Zhang, W., Crittenden, J., Minakata, D., Chen, Y. and Wang, P. Effects of inorganic electron donors in photocatalytic hydrogen production over Ru/(CuAg) 0.15 In0. 3Zn1. 4S2 under visible light irradiation. Journal of Renewable and Sustainable Energy, 2014, 6, 033131.
  11. Zhang, G., Zhang, W., Minakata, D., Chen, Y., Crittenden, J. and Wang, P. The pH effects on H2 evolution kinetics for visible light water splitting over the Ru/(CuAg)0.15In0.3Zn1.4S2 photocatalyst. International Journal of Hydrogen Energy, 2013, 38, 11727-11736.
  12. Zhang, G., Zhang, W., Crittenden, J.C., Chen, Y., Minakata, D. and Wang, P. Photocatalytic hydrogen production under visible-light irradiation on (CuAg)0.15In0.3Zn1.4S2 synthesized by precipitation and calcination. Chinese Journal of Catalysis, 2013, 34, 1926-1935.
  13. Zhang, G., Zhang, W., Wang, P., Minakata, D., Chen, Y. and Crittenden, J. Stability of an H2-producing photocatalyst (Ru/(CuAg)0.15In0.3Zn1.4S2) in aqueous solution under visible light irradiation. International Journal of Hydrogen Energy, 2012, DOI: 10.1016/j.ijhydene.2012.11.033.
  14. Ge, S., Agbakpe, M., Zhang, W., Kuang, L., Wu, Z. and Wang, X. Recovering Magnetic Fe3O4-ZnO Nanocomposites from Algal Biomass Based on Hydrophobicity Shift under UV Irradiation. ACS Applied Materials & Interfaces, 2015, 7, 11677–11682.
  15. Ge, S., Agbakpe, M., Zhang, W. and Kuang, L. Heteroaggregation between PEI-Coated Magnetic Nanoparticles and Algae: Effect of Particle Size on Algal Harvesting Efficiency. Acs Applied Materials & Interfaces, 2015, 7, 6102-6108.
  16. Ge, S., Agbakpe, M., Wu, Z., Kuang, L., Zhang, W. and Wang, X. Influences of Surface Coating, UV Irradiation and Magnetic Field on the Algae Removal Using Magnetite Nanoparticles Environmental Science & Technology, 2014, 49 1190–1196.
  17. Agbakpe, M., Ge, S., Zhang, W., Zhang, X. and Kobylarz, P. Algae harvesting for biofuel production: Influences of UV irradiation and polyethylenimine (PEI) coating on bacterial biocoagulation. Bioresource Technology, 2014, 166, 266–272.
  18. Zhang, W., Zhang, W., Zhang, X., Amendola, P., Hu, Q. and Chen, Y. Characterization of dissolved organic matters responsible for ultrafiltration membrane fouling in algal harvesting. Algal Research, 2013, 2, 223-229.

Nitrogen Solubility in Marine Animals

We were very fortunate to receive the Hamilton syringe grant, as it allowed us to acquire syringes for two projects: for the gas chromatograph, which in our lab is used for fatty acid analysis of animal tissues (from a wide range of species, from zooplankton to storm petrels, penguins to sea turtles, lobsters to bowhead whales!) and for our nitrogen apparatus, which we use to measure the solubility of nitrogen gas in oils extracted from the tissues of diving animals.

Many students, both undergraduate and graduate, benefited directly from this grant as they used either the gas chromatograph or the nitrogen apparatus. Data resulting from these studies was presented at several conferences, including the Society for Integrative and Comparative Biology in Austin TX (2014 and 2015), the Southeast and Mid Atlantic Marine Mammal Symposium (2014 and 2015), and the Society for Marine Mammalogy. The most significant scientific outcome we had this year was the recent publication of my M.Sc. student’s work in the Journal of Experimental Biology, in which we describe the high nitrogen solubility in the special echolocation tissues of toothed whales, and the fact that this might make them vulnerable to gas emboli in these tissues if their normal diving behavior is disrupted.

Gina L. Lonati, Andrew J. Westgate, D. Ann Pabst and Heather N. Koopman.

Nitrogen solubility in odontocete blubber and mandibular fats in relation to lipid composition. J Exp Biol 2015 218:2620-2630.

The paper was selected by the J. Exp. Biol. editors as one to highlight in their “Inside JEB” section of the issue:  http://jeb.biologists.org/content/218/16/2491

and then was subsequently picked up by National Geographic online: