Carolina Burgos-Vega and the University of Texas at Dallas is pioneering a new technique for stimulating the meninges in order to study the pathophysiology of migraine headache. Migraine headache is the most prevalent neurological disorder but the pathophysiology contributing to the development and progression of the condition is not well understood. Current pharmacological treatments are inadequate at managing the complex symptomology experienced by migraine sufferers and thus new therapeutics are needed. Increasing amounts of evidence suggest that nociceptor activation in the meninges plays a role in migraine pain. Identification of mechanisms leading to activation/sensitization of these neurons may provide targets for novel migraine therapeutics. Previous work in rat models of migraine pain has found that stimulation of the dura produces afferent input to the central nervous system leading to headache-like behaviors (cutaneous allodynia). However, development of a mouse model of headache using dural stimulation would allow the use of genetic tools for target identification that do not exist in rats.
Hamilton Neuros syringes will allow them to inject onto the surface of the meninges (dura) of mice, using the cranial suture junction as an entry point. At 8 weeks of age the suture junctions are not fused therefore with a controlled penetration depth and no dead space of volume will allow for the precision needed to preserve the integrity of the tissue. Unlike previous studies conducted in rats we will not utilize a cannula, which requires substantial recovery. The Neuros line of syringes will allow them to change the needle depth based on the size and weight of animals. Thus customization is based of the animal itself and conducted by the experimenter.
What makes the University of Texas at Dallas laboratory unique is the number of students that they mentor. At any given time they will have upwards of 30 undergraduate students working within the lab. The other graduate students take pride in the innovation and inspiration that introducing so many new students to the research community brings. They can see themselves in the young minds and strive to cultivate success both for their personal and professional growth, as well as their own. Many of their students have gone off to professional or graduate school, with some even deciding to stay in Neuroscience.
They hope that by exposing undergraduates to their migraine studies they can instill in our future leaders the the value of preclinical research, while also engaging and empowering students through hands-on learning in the lab.
Like many other colleges, due to budget limitations, the Organic Chemistry students at Cerritos Community College in Norwalk, CA were using plastic, disposable syringes. Because plastic does not work well with organic chemicals, the students were using medicine droppers to count by drops, when transferring liquids.
Cerritos Community College is proud of their very high standard of teaching within their Organic Chemistry courses. Rigorous training in their labs requires the use of good-quality syringes/needles that will enhance their students’ experience in the labs. Good hands-on training is a must for their pre-med, pre-pharmacy, pre-dental, and future researcher students.
At the end of their one-year course, their students are required to take the national American Chemical Society standardized exam. An amazing 40% of their students achieve 90th percentile or above. That means that 40% of their students perform better than 90% of all the Organic Chemistry students nationwide.
Hamilton Company is happy to award $1,000 worth of high quality syringes that will enhance the lab experience for their students. Congratulations and continued success to Cerritos Community College!
spinal muscular atrophy (SMA) using Hamilton Microliter 700 syringes.
The laboratory of Professor Thomas Gillingwater at the University of Edinburgh is developing novel therapeutics for the treatment of the childhood neuromuscular disease, spinal muscular atrophy (SMA). As the genetic defects of SMA are well characterized, mouse models of SMA have been developed which are utilized to develop treatment strategies for this currently incurable condition.
In Professor Gillingwaters lab, Hamilton syringes and needles are used to accurately deliver small molecule compounds and gene therapy vectors via a variety of injection techniques (intravenous, intraperitoneal, intramuscular, subcutaneous, and intrauterine to the SMA mouse model and the potential therapeutic potential of a drug assessed by behavioral, histological and molecular analysis. This strategy has been employed to great success, with recent identification of novel pathways that can improve the SMA phenotype in animal models (Wishart et al., 2015, JCI; 124(4):1821-34; Powis et al., 2016 JCI Insight; 1(11):e87908).
Research projects in the Gillingwater lab, are mainly funded by a not-for-profit UK charity that supports research into this devastating condition: the SMA Trust (http://www.smatrust.org/).
The Boston College project will utilize Designer Receptor Exclusively Activated by Designer Drug (DREADD) constructs. The neuronal alterations induced by the DREADD virus will allow for control of the activity of the specific circuits infected through subsequent drug delivery. This circuit-level modulation will occur during experiments designed to test how defined neuronal populations govern various aspects of social behavior.
into fetal brain matter to alter miRNA and lncRNA expression at embryonic day 14 in the mouse. Fetal pups are returned to the mouse dam and then are allowed to finish developing until birth. We will then assess the impact of altering specific miRs and lncRNAs which are known to regulate the glucocorticoid pathway. The ncRNAs will be directly injected into the fetal brain and this must be done in a small volume so that the fetal mice will survive the procedure. Once we have this procedure working we will train graduate students at the University of New Mexico, Neuroscience department.Learn more about Neuros syringes